Immune and inflammatory responses mediated by cytokines, play important roles in the
pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines
such as IL-4 and IL-13. These two cytokines use common receptors for signaling that lead to identical immunological effects and regulation of the Th1/Th2 balance. The aim of this study was to determine whether patients with allergic asthma display overexpression of IL-4 and IL-13 genes.
Using RT-PCR, we examined the expression of IL-4 and IL-13 genes in twenty asthmatic cases
and twenty normal individuals. Total levels of serum IgE and IL-4 were also determined by ELISA method.
Expression of IL-13 gene in 70% of patients with allergic asthma was higher than controls
(P=0.01). There was no correlation between the expression of IL-13 gene and total level of serum IgE (P=0.07). Expression of IL-4 gene was detected in 30% of the patients and none of the normal individuals as determined by RT-PCR (P=0.01). Mean of serum IgE levels in patients and controls were 84.9 IU/ml and 62.2 IU/ml, respectively. Level of serum IgE was more than 100 IU/ml in 30% of patients (P=0.03). Mean of serum IL-4 levels in patients and controls were 15.73 pg/ml and 13.07 pg/ml, respectively. There was a relation between levels of serum IgE and IL-4 in 73% of cases. The results showed that there was a correlation between the expression of IL-4 gene and the level of serum IL-4. Levels of serum IgE and IL-4 were considerably higher in asthmatics than nonasthmatic
controls.
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原文发布时间:2006/12/16
引用本文:
Jalil Tavakkol Afshari;Reza Farid Hosseini;Sara Hosseini Farahabadi;Farhad Heydarian;Mohammad Hossein Boskabady;Rasoul Khoshnavaz;Alireza Razavi;Ehsan Ghayoor Karimiani;Gholamreza Ghasemi.Association of the Expression of IL-4 and IL-13 Genes,IL-4 and IgE Serum Levels with Allergic Asthma.http://hftc.firstlight.cn/View.aspx?infoid=924842&cb=nijuyanxg.
发布时间:2006/12/16.检索时间:2024/12/15