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检测结核分枝杆菌蛋白抗原b夹心ELISA的建立及其应用
结核分枝杆菌 蛋白抗原b夹心ELISA 建立应用
2013/9/17
建立定量检测脑脊液中结核分枝杆菌蛋白抗原b的夹心ELISA, 以便用于诊断结核性脑膜炎。方法 制备抗结核分枝杆菌蛋白抗原b鼠源性单克隆抗体(mAb)及兔多克隆抗体; 以单克隆抗体为包被抗体, 多克隆抗体为检测抗体, 建立检测结核分枝杆菌蛋白抗原b的双抗体夹心ELISA;应用此方法检测正常人（n=6）、 非结核性脑膜炎患者（n=26）及临床确诊结核性脑膜炎患者（n=42）的脑脊液标本中蛋白抗原b的含...
梅毒是由梅毒螺旋体(Treponema pallidum, TP)引起的一种性传播疾病, 近年来我国的梅毒发病率有逐年上升的趋势。梅毒特异性抗体的血清学检查是梅毒诊断的重要依据。传统血清学检测方法有梅毒螺旋体血球凝集试验(TPHA)和梅毒螺旋体酶联免疫吸附试验(TP-ELISA)[1] 。长期以来国内外都在不断探讨更灵敏或更快速的梅毒血清学检测技术。近年来发展起来的流式微球技术 (flow mi...
Evaluation of Dot-ELISA Method Using Excretory-Secretary Antigens of Fasciola hepatica in Laboratory Diagnosis of Human Fasciolosis
Dot-ELISA Excretory-secretory antigen Fasciola hepatica Diagnosis
2010/2/4
Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. In this study, Dot-ELISA, one of the simplest and the most sensitive tests in th...
Comparative Evaluation of Fast Enzyme Linked Immunosorbent Assay (Fast-ELISA) and Standard-ELISA For The Diagnosis Of Human Hydatidosis
Hydatidosis Fast- ELISA Standard-ELISA
2010/2/4
Fast enzyme linked immunosorbent assay (Fast-ELISA) was compared with the standard ELISA for the diagnosis of human hydatidosis. Seventy serum samples including 30 from hydatidosis patients (surgicall...