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Detection of the SRY Gene in a 46,XY Phenotypic Female by the PCR-SSCP Method
PCR-SSCP Sex reversal SRY 46 XY Female
2009/6/30
The short arm of the human Y chromosome determines maleness, initiating the development of the testes. Mutations and deletions in this region affect gonadal differentiation, causing primary sex revers...
Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration
DNA concentration spot density analysis ethidium bromide
2009/6/19
A commonly used method to measure the amount of DNA in nucleic acid preparations is to use UV irradiation. However, if the DNA sample contains impurities, measuring UV irradiation is misleading. A qui...
Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method
DNA extraction Mycobacterium tuberculosis PCR
2009/5/20
Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boil...