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Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry
Methanogen Anaerobic Digester 16S rRNA mcrA Methanobacterium formicicum
2016/5/20
A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, ...
Lactic Acid Bacteria in Total Mixed Ration Silage Containing Soybean Curd Residue: Their Isolation, Identification and Ability to Inhibit Aerobic Deterioration
Soybean Curd Residue–Containing TMR Silage Aerobic Deterioration Lactic Acid Bacteria Synergistic Effect
2016/5/20
We investigated the effects of the predominant lactic acid bacteria (LAB) on the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage containing soybean curd residue (...
Urinary Catecholamine and Cortisol Responses of Japanese Shorthorn Cows to Social Isolation
Urinary Cortisol Urinary Catecholamines Social Isolation
2016/5/3
This study was performed to investigate the use of urinary catecholamines to monitor changes in the activity of the sympathetic nervous system and to determine the relationship of urinary cortisol and...
Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Spermatogonial Stem Cell SSC Pluripotency Long Term Culture Pig
2016/4/29
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for ...
Strong Reproductive Isolation between Closely Related Tropical Sea Urchins (genus Echinometra)’
Reproductive Isolation Closely Related
2015/12/17
Morphological, mitochondrial DNA, and single-copy nuclear DNA differences show
that the tropical sea urchin Echinometra mathaei is composed of at least four
independent gene pools. Evolutionary di...
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
2016/5/18
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, whi...
Isolation and Characterization of Novel Denitrifying Bacterium Geobacillus sp. SG-01 Strain from Wood Chips Composted with Swine Manure
Geobacillus thermodenitrificans Nitrates Nitrites Swine Manure Wood Chips
2016/5/12
Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollut...
Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability
Swine Intestinal Microbiota Enzyme-linked Immunosorbent Assay
2016/5/9
The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb ...
Isolation and Characterization of Parthenogenetic Embryonic Stem (pES) Cells Containing Genetic Background of the Kunming Mouse Strain
Mouse Parthenogenetical Activation Parthenogenetic Embryonic Stem (pES) Cells Kunming Mouse (Mus musculus Km) Genetic Background
2016/5/5
Parthenogenetic embryonic stem (pES) cells could provide a valuable model for research into genomic imprinting and X-linked diseases. In this study, pES cell lines were established from oocytes of hyb...
Validation of Methods for Isolation and Culture of Alpaca Melanocytes: A Novel Tool for In vitro Studies of Mechanisms Controlling Coat Color
Alpaca Alpha Melanocyte Stimulating Hormone Coat Color Melanocortin 1 Receptor Melanocyte Pigmentation
2016/5/4
The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat ...
Isolation, Expression Pattern, Polymorphism and Association Analysis of Porcine TIAF1 Gene
TIAF1 Porcine Real-time PCR Carcass Traits Meat Quality Traits Deletion Mutation
2016/4/29
TIAF1 is a TGF-1-induced anti-apoptotic factor that plays a critical role in blocking TNF (tumor necrosis factor) cytotoxicity in mouse fibroblasts and participates in TGF--mediated gr...
Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts
Parthenogenetic Activation Oocyte Embryonic Stem Cells Porcine
2016/4/26
These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-...
Isolation and Cloning of Porcine SLC27A2 Gene and Detection of Its Polymorphism Associated with Growth and Carcass Traits
Solute Carrier Family 27 Member 2 Pig Polymorphism
2016/4/26
The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key rol...
Isolation of an Oocyte Stimulatory Peptide from the Ovarian Follicular Fluid of Water Buffalo (Bubalus bubalis)
Follicular Fluid Peptide Oocyte IVM Buffalo
2016/4/20
Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biologic...
Isolation, In vitro Antibacterial Activity, Bacterial Sensitivity and Plasmid Profile of Lactobacilli
Lactobacilli Competitive Exclusion Antibacterial Activity Plasmid
2016/4/20
The present research work was conducted to evaluate the beneficial effects as well as the safety aspects of lactobacilli as probiotic. Lactobacilli were isolated from poultry faecal samples, feed samp...