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Effects of Candida norvegensis Live Cells on In vitro Oat Straw Rumen Fermentation
Rumen Fermentation Yeast Oat Straw Methane
2016/5/19
This study evaluated the effect of Candida norvegensis (C. norvegensis) viable yeast culture on in vitro ruminal fermentation of oat straw. Ruminal fluid was mixed with buffer solution (1:2) and anaer...
Aerobic Uptake of Cholesterol by Ergosterol Auxotrophic Strains in Candida glabrata & Random and Site-Directed Mutagenesis of ERG25 in Saccharomyces cerevisiae
Ergosterol Candida glabrata ERG25
2015/5/22
Candida albicans and Candida glabrata are opportunistic human pathogens that are the leading cause of fungal infections, which are increasingly becoming the leading cause of sepsis in immunosuppressed...
Candida sp.脂肪酶的纯化及其性质
脂肪酶 纯化 层析
2009/11/16
采用简单的两步法-离子交换层析和疏水层析法,对Candida sp. 99-125脂肪酶进行了纯化,比活提高了10.0倍,达到27200 U/mg,回收率为35.5%. SDS-PAGE电泳分析显示该酶的分子量约为38 kDa. 酶学性质研究表明,该酶最适反应温度为40℃,最适反应pH值为8.5,在室温下具有良好的稳定性. 钙离子和Tween80能够促进提高脂肪酶的活性,而铁离子、铜离子和SDS对...
假丝酵母Candida sp.发酵生产脂肪酶培养条件的优化
脂肪酶 Candida sp. 假丝酵母
2009/11/4
研究了碳源和氮源种类、碳源浓度以及起始pH 值对假丝酵母Candida sp.菌株发酵生产脂肪酶的影响.结果显示,最适碳源为橄榄油,氮源为酵母膏,橄榄油浓度为2%,起始pH 值为6.0.应用响应面试验设计对酵母膏、橄榄油和起始pH 值进行培养基优化,结果表明,优化后的培养基配方为:橄榄油2.582%,酵母膏0.276%,K2HPO4 0.1%,MgSO4·7H2O 0.01%,NaCl 0.05%...
假丝酵母Candida sp.发酵生产脂肪酶培养条件的优化
脂肪酶 假丝酵母 响应面分析 发酵
2009/11/3
研究了碳源和氮源种类、碳源浓度以及起始pH 值对假丝酵母Candida sp.菌株发酵生产脂肪酶的影响.结果显示,最适碳源为橄榄油,氮源为酵母膏,橄榄油浓度为2%,起始pH 值为6.0.应用响应面试验设计对酵母膏、橄榄油和起始pH 值进行培养基优化,结果表明,优化后的培养基配方为:橄榄油2.582%,酵母膏0.276%,K2HPO4 0.1%,MgSO4·7H2O 0.01%,NaCl 0.05%...
Study takes aim at opportunistic fungal pathogens--Research provides genome sequences and critical analyses of key Candida species
fungal pathogens genome sequences critical analyses Candida species
2009/6/22
In what represents one of the largest comparative genomics studies to date, scientists have cracked the genetic code of several fungal species that cause bloodstream infections in patients with suppre...
Candida rugosa脂肪酶同工酶的分离及选择固定化
CRL同工酶 分离 界面亲和层析 选择性固定化
2008/11/4
采用“界面亲和层析”,从商品Candida rugosa脂肪酶(CRL)中分离到三个同工酶(CRL-1、CRL-2和CRL-3),它们在水-有机溶剂双液相体系中催化(R,S)-萘普生甲酯的不对称水解反应,具有不同的立体选择性.分析表明:CRL-1和CRL-2上不同程度地非共价结合有小分子的酸性化合物,阻碍了其活性位点处疏水腔的完全开放;CRL-3上不含有该小分子酸性化合物,活性位点处疏水腔可处于完...
期刊信息
篇名
cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol
语种
英文
撰写或编译
撰写
作者
Cao YY,Cao YB,Xu Z,Ying K,Li Y,Xie Y,Zhu ZY,Chen WS,Jiang YY*
第一作者单位
...
期刊信息
篇名
cDNA Array Analysis of Differential Expression Change in Virulence-related Genes During the Development of Resistance in Candida albicans
语种
英文
撰写或编译
撰写
作者
Xu Z,Cao YB,Zhang JD,Cao YY,Gao PH,W...
Effects of Combination of Nitrate with β1-4 Galacto-oligosaccharides and Yeast (Candida kefyr) on Methane Emission from Sheep
Rumen Methanogenesis Nitrate 1-4 Galacto-oligosaccharide Candida kefyr Methaemoglobin
2016/4/14
The objective of the present study was to determine whether 1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitra...