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中国科学院动物研究所专利：Recombinant broad-spectrum Metarhizium and production method and application thereof
中国科学院动物研究所 专利 broad-spectrum Metarhizium
2024/5/31
本发明提供了一种下调或不表达单胺氧化酶的重组广谱绿僵菌， 或其中色胺含量高于野生型广谱绿僵菌，重组广谱绿僵菌是菌株本身，菌株后代，菌株产生的分生孢子，菌株产生的菌丝体或它们的任意组合。 从重组广谱绿僵菌中敲除单胺氧化酶基因，显著提高了绿僵菌中色胺的浓度， 从而显著提高其杀虫效率，使野生型广谱绿僵菌的半致死时间LT50从7.33±0.445天缩短到6.136±0.488天。 本发明的重组广谱绿僵菌对...
中国科学院动物研究所专利：Recombinant broad-spectrum Metarhizium and its use for promoting plant root growth
中国科学院动物研究所 专利 重组 广谱绿僵菌 植物根系生长
2024/5/30
本发明涉及一种重组广谱绿僵菌及其在促进植物根系生长中的应用，属于农业生物技术领域。 本发明的重组广谱绿僵菌表达下调的单胺氧化酶或不表达单胺氧化酶，能够促进植物根的生长。
中国科学院动物研究所专利：Recombinant Metarhizium acridum, preparation method therefor and use thereof
中国科学院动物研究所 专利 Metarhizium acridum
2024/5/30
中国科学院动物研究所专利：Recombinant Metarhizium acridum, preparation method therefor and use thereof。
Identification of candidate genes for key fibre-related QTLs and derivation of favourable alleles in Gossypium hirsutum recombinant inbred lines with G. barbadense introgressions
海岛棉 基因组组分 遗传作图群体
2024/5/17
该研究以渗入了海岛棉基因组组分的优质种质鲁原343为亲本，构建了包含239个重组自交系的遗传作图群体，利用SLAF标签构建了高密度遗传图谱，结合7个环境的表型数据，共鉴定104个纤维品质和产量性状QTLs，其中66个QTL共定位于19个QTL簇中。该研究团队通过进一步对鲁原343进行全基因组深度测序，解析鲁原343的遗传组成及其对纤维相关性状的遗传贡献，发现来自G. hirsutum races（...
Genome-wide quantitative trait loci reveal the genetic basis of cotton fiber quality and yield-related traits in a G.hirsutum recombinant inbred line population
0-153 sGK9708 棉花产量 品质性状
2024/5/17
利用0-153和sGK9708为亲本构建的RIL群体为材料，通过全基因组QTL挖掘明确了棉花产量及品质性状的相关性及其与QTL簇加性效应方向的关系，初步揭示了棉花产量纤维品质性状负相关形成的遗传基础，为进一步利用标记辅助选择、基因组编辑等技术同步改良棉花纤维品质和产量提供了重要的基因资源和位点信息。该研究以0-153*SGK9708为亲本构建的含有196个重组自交系群体为材料，在黄河流域及西北内陆...
新乡学院生命科学技术学院基因工程课件 Expression and Modification of Recombinant Proteins
新乡学院生命科学技术学院 基因工程 课件 Expression and Modification of Recombinant Proteins
2017/7/17
新乡学院生命科学技术学院基因工程课件 Expression and Modification of Recombinant Proteins。
新乡学院生命科学技术学院基因工程课件 Screening and Identification of Recombinant Clones
新乡学院生命科学技术学院 基因工程 课件 Screening and Identification of Recombinant Clones
2017/7/17
新乡学院生命科学技术学院基因工程课件 Screening and Identification of Recombinant Clones。
Quantitative trait loci for resistance to Sharp Eyespot (Rhizoctonia cerealis) in recombinant inbred wheat lines from the cross Niavt 14 × Xuzhou 25
genetic map molecular marker QTL Rhizoctonia cerealis Sharp Eyespot wheat
2016/12/8
This paper studies QTL (quantitative trait locus) resistance to Sharp Eyespot in wheat using a genetic population of the recombinant inbred line (RIL) hybridized from Niavt 14 and Xuzhou 25. Based on ...
Effects of weekly administration of pegylated recombinant human OB protein on appetite profile and energy metabolism in obese men
Obesity leptin OB protein
2016/6/14
Results of leptin administration in mice, rats, and
humans provide a rationale for therapeutic augmentation of circulating
leptin (OB protein) concentrations in obese humans;
this may reduce food i...
Purification and quantification of lactoperoxidase in human milk with use of immunoadsorbents with antibodies against recombinant human lactoperoxidase
Lactoperoxidase myeloperoxidase SDS-PAGE
2016/6/13
Two heme-containing peroxidases, secretory lactoperoxidase
and leukocyte-derived myeloperoxidase, which play
host defense roles through antimicrobial activity, were previously
identified in human c...
Frictional Drag and Electrical Manipulation of Recombinant Proteins in Polymer-Supported Membranes
Polymers Lipids Recombinant Fusion Proteins Green Fluorescent Proteins Membrane Proteins Electrophoresis Membranes,Artificial Osmosis Histidine Models, Chemical
2016/5/24
We establish a lipid monolayer supported by a polymer interface that offers advantages over conventional solid-supported membranes for determining the frictional drag at the membrane-protein interface...
X-ray Crystal Structure of a Mutant Recombinant Human Myoglobin at 2.8Å Resolution
Humans Myoglobin Recombinant Proteins X-Ray Diffraction Protein Conformation Mutation Molecular Structure
2016/5/23
We have grown crystals in trigonal space group P 3 2 21 of a mutant human myoglobin, aquomet form, in which lysine at position 45 has been replaced by arginine and cysteine at position 110 has been re...
Electrostatic Interactions in Wild Type and Mutant Recombinant Human Myoglobins
SUBSTANCE-P GABA PROLACTIN ANTERIOR PITUITARY HYPOTHALAMIC FRAGMENTS ANTI-SUBSTANCE-P SERUM
2016/5/20
Residue Val68 in human myoglobin has been replaced by Asn, Asp, and Glu with site-directed mutagenesis. Purified proteins were characterized by isoelectric focusing and by absorption, CD, and NMR spec...
Ligand and Proton Exchange Dynamics in Recombinant Human Myoglobin Mutants
Mb myoglobin n.m.r. nuclear magnetic resonance fwhm full width at half maximum TSP sodium 3-trimethyl silyl propionate DSS 4,4-dimethyl-4-sila pentane-1-sulfonate
2016/5/20
Site-specific mutants of human myoglobin have been prepared in which lysine 45 is replaced by arginine (K45R) and aspartate 60 by glutamate (D60E), in order to examine the influence of these residues ...
Effects of Buried Ionizable Amino Acids on the Reduction Potential of Recombinant Myoglobin
Buried Ionizable Amino Acids Reduction Potential Recombinant Myoglobin
2016/5/20
The temperature dependences of the reduction potentials (E degrees') of wild-type human myoglobin (Mb) and three site-directed mutants have been measured by the use of thin-layer spectroelectrochemist...