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The pattern of LH release in the adult ram influences the testicular steroidogenic response to individual LH pulses and is regulated by testosterone negative feedback
LH release adult ram influences testicular steroidogenic individual LH pulses
2009/2/23
Two experiments were conducted with adult intact rams (approximately 58 kg in body weight) in the nonbreeding season to investigate interrelationships between LH and testosterone secretion. In Exp...
Steroidogenesis and testosterone metabolism in cultured principal cells from the ram epididymis
Steroidogenesis testosterone metabolism cultured principal cells ram epididymis
2009/2/23
To determine if ram principal cells can synthesize or metabolize testosterone, or metabolize other steroids present in rete testis fluid, principal cells from the initial segment, central caput, and...
Effect of adrenal steroids on testosterone and luteinizing hormone secretion in the ram
adrenal steroids testosterone luteinizing hormone secretion ram
2009/2/23
The effects of adrenal steroids on testosterone and LH secretion and changes in serum cortisol levels in response to treatments were studied in the ram. Acute administration of synthetic ACTH (10 mi...
Reversible harmless interruption of testicular blood supply in the ram
Reversible harmless interruption testicular blood ram
2009/2/23
An effective method of interrupting testicular blood flow temporarily and repeatedly in the ram has been developed. Blockade of flow has been achieved mechanically by an inflatable occluder placed a...
Culture of principal cells from the ram epididymis
principal cells ram epididymis morphology
2009/2/20
A procedure was developed to isolate and culture principal cells from the initial segment, central caput, distal caput, and proximal corpus epididymidis. The morphology of cultured cells and of cell...
Human and Ram Seminal Plasma Both Contain a Calcium-Dependent Regulator Protein
Spermatozoa initiation of motility Ca2+-dependent regulator Ca2+-ATPase epididymis
2009/2/19
Biochemical aspects of post-testicular sperm maturation have been studied. Partial purification of either human or ram seminal plasma yields a heat-stable, acidic protein which is a Ca2+-dependent...
Fertility and its relationship to motility characteristics of spermatozoa in ewes after cervical, transcervical, and intrauterine insemination with frozen-thawed ram semen
Proline glycine betaine sperm cryopreservation
2009/2/18
The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen...
Improvement of cryopreserved ram sperm heterogeneity and viability by addition of seminal plasma
cryopreserved ram sperm heterogeneity seminal plasma
2009/2/17
The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simulta...
Polyamines in ejaculated ram spermatozoa and their relationship with sperm motility
Ram ejaculated spermatozoa polyamines sperm motility
2009/2/10
Intracellular polyamine levels in ejaculated spermatozoa and seminal fluid from rams were determined by fluorescent spectroscopy of their dansyl derivatives. Relationships between the sperm polyamin...
Endocytosis of androgen-binding protein, clusterin, and transferrin in the efferent ducts and epididymis of the ram
Testicular proteins uptake excurrent ducts
2009/2/7
The amount of androgen-binding protein (ABP) in luminal fluid from the central caput epididymidis of the ram, on a per sperm basis, remains the same as that in rete testis fluid (RTF) entering the...
Proteins in luminal fluid of the ram excurrent ducts：changes in composition and evidence for differential endocytosis
sperm proteins androgen-binding protein endocytosis rete testis fluid ductuli efferentes
2009/2/6
Rete testis fluid (RTF) and luminal fluid collected by micropuncture at selected epididymal sites were analyzed to characterize the spectrum of proteins and to quantify the net gain or loss of total...
Effect of scrotal insulation on clusterin-positive cells in ram semen and their relationship to semen quality
Clusterin abnormal spermatozoa semen analysis
2008/12/29
Scrotal contents of 2 rams were insulated for 96 hours and the fraction (as a percentage) of clusterin-positive cells (CPCs) and its relationship to semen quality was investigated. Semen collection ...
Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system
ram spermatozoa X and Y chromosome centrifugal countercurrent aqueous two-phase system
2008/12/25
The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic o...
Assessment of the acrosomal status of ram spermatozoa by RCA lectin-binding and partition in an aqueous two-phase system
acrosomal status ram spermatozoa RCA lectin-binding partition aqueous two-phase system
2008/12/25
The acrosome reaction is an important marker for sperm function. Because different laboratory techniques may be used to detect this exocytotic process, the objective of this study was to investiga...
Effect of Seminal Plasma Fractions From Entire and Vasectomized Rams on the Motility Characteristics, Membrane Status, and In Vitro Fertility of Ram Spermatozoa
Seminal Plasma Fractions Entire Vasectomized Rams Motility Characteristics Membrane Status
2008/12/24
Whole seminal plasma from ram semen collected before and after vasectomy was separated into 2 fractions, supernatant and pellet of vesicles, and their protein profiles characterized by one-dimens...