搜索结果: 1-15 共查到“兽医科学基础学科 polymorphism”相关记录16条 . 查询时间(0.078 秒)
Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits
milk yield fat percentage dairy goat SNP PCR-RFLP SSCP additive effect
2015/6/24
Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the...
Testing different single nucleotide polymorphism selection strategies for prediction of genomic breeding values in dairy cattle based on low density panels
3K chip genomic selection prediction single nucleotide polymorphism
2015/6/24
In human and animal genetics dense single nucleotide polymorphism (SNP) panels are widely used to describe genetic variation. In particular genomic selection in dairy cattle has become a routinely app...
Polymorphism identification in the goat MSTN gene and association analysis with growth traits
MSTN gene polymorphisms growth traits linkage disequilibrium
2015/6/9
The myostatin (MSTN) gene was studied as a candidate genetic marker for growth traits. We investigated polymorphisms of the MSTN gene in 664 individuals from four goat populations and applied PCR-SSCP...
Analysis of polymorphism in the porcine TLR4 gene and its expression related to Escherichia coliF18 infection
pigs TLR4 gene polymorphism expression profile ETEC F1
2015/6/9
The genetic variation in exon 1 of the TLR4 gene was detected among a total of 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous pig breeds. The expression of TL...
High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle
HRM analysis PCR-RFLP K232A polymorphism
2015/6/9
PCR-RFLP analysis is a common method for genotyping the DGAT1 K232A polymorphism in cattle. Our purpose was to develop a high resolution melting (HRM) assay in order to genotype the polymorphic allele...
The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds
sheep FABP4 gene polymorphism intramuscular fat
2015/6/9
The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality trait...
A single nucleotide polymorphism in exon 3 of the myostatin gene in different breeds of domestic pigeon (Columba livia var. domestica) 32
myostatin polymorphism synonymous mutation muscle growth
2015/4/17
Myostatin is considered to be one of the most powerful negative regulators of muscle growth. The lack of a functional myostatin or its mutation can result in uncommon musculature like “double-muscling...
Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk
lactoferrin polymorphism somatic cell count bovine
2015/3/5
The study included 124 Polish Black-and-White dairy cows of various share of the Holstein-Friesian (HF) breed. Lactoferrin (LTF) gene polymorphism was obtained with PCR-RFLP method using EcoRI enzyme....
Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene
RFLP PRRSV porcilis pyrsvac ORF5
2015/1/26
Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of re...
New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
chimeric chicken germline mitochondrial DNA D-loop region single nucleotide polymorphism sperm
2009/7/31
Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such...
选择作用下家鸡淀粉酶的遗传多态性研究――基因频率分化、相关效应估计和标记辅助选择试验Plasma Amylase Polymorphism under Selection
淀粉酶 遗传多态性 遗传标记 选择试验
2008/1/30
摘要对“三黄”鸡4个品系的血液淀粉酶(Amy-1)基因频率进行了估计, 结果表明: Amy-1基因频率具有明显的品系特异性, 父系Amy-1A高; 母系Amy-1B频率高。人工选择可能是导致两个具有相同遗传来源的品系（Ⅱ系和Ⅲ系）Amy-1基因频率发生分化的原因。利用线性模型估计了Amy-1基因的加性效应，结果表明：Amy-1B有利于产蛋性能, 而Amy-1A有利于体重和蛋重的提高。标记辅助选择试...
多浪羊MHC-DRB3基因座的PCR-RFLP多态性分析Polymorphism Analysis of MHC-DRB3 Gene in Dolang Sheep with PCR-RFLP
多浪羊 MHC-DRB3 分子遗传多态性 PCR-RFLP
2008/1/10
摘要 主要组织相容性复合体（MHC）是由紧密连锁的高度多态的基因位点所组成的染色体上的一个遗传区域，它在动物机体的免疫系统中发挥着非常重要的作用。应用PCR-RFLP技术首次对多浪羊的MHC-DRB3的外显子2进行分子遗传多态性检测与分析。结果显示，多浪羊MHC-DRB3基因的外显子2在TaqⅠ、PstⅠ和HaeⅢ酶切位点存在多态，其酶切位点分别由2、2和6种共显性等位基因控制。综合3种酶切结果，...
蒙古山羊和哈萨克山羊GOLA-DRB3基因的HaeⅢ酶切多态性分析Polymorphism Analysis of the GOLA-DRB3 Gene Digested with HaeⅢ in Mongolian Goat and Kazakh Goat
蒙古山羊 哈萨克山羊 GOLA-DRB3 PCR-RFLP
2008/1/10
摘要
采用限制性内切核酸酶HaeⅢ对蒙古山羊和哈萨克山羊GOLA-DRB3基因外显子2的285bp扩增产物进行了PCR-RFLP多态性分析，共检测到17种基因型，由A、B、C、D、E、F和H等7个复等位基因控制；通过酶切图谱分析发现蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的154、168和220位碱基表现出多态性。并对基因型频率和等位基因频率进行了统计分析，结果表明，GOLA-DR...
猪白细胞Ⅱ类抗原基因多态性研究进展Advances in Allelic Polymorphism at Class Ⅱ of the Pig MHC(SLA)
猪 SLAⅡ类基因 RFLP 多态性
2007/12/31
摘要本文对SLAⅡ类基因的染色体图谱定位、分子结构、基因分型及其多态性的研究进展作了介绍。重点介绍了SLAⅡ类基因的RFLP基因分型及其多态性研究。猪的主要组织相容性复合物Ⅱ类抗原基因座位存在不同程度的等位基因多态性。RFLP技术是一种简便而快捷的SLAⅡ类等位基因分型方法。SLAⅡ类基因的多态性和抗原肽结合位点密切相关，这些位点能结合处理过的抗原，然后递呈给T细胞。另外，在编码β1链的SLA-D...
摘要
利用4个微卫星标记（OarFCB11，OarAE101，McM218，McM38）对波尔山羊、太行山羊和河北奶山羊的等位基因频率、群体多态信息含量、有效等位基因数、杂合度和遗传距离进行了遗传检测，并测定了波尔山羊与河北奶山羊及太行山羊的杂交效果。结果表明：4个微卫星标记在波尔山羊、太行山羊和河北奶山羊3个品种中存在多态性，可以用于山羊遗传多样性的评估；从不同品种来看，太行山羊的遗传变异程度...