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Histological characteristics of globular shaped pollen callus (globules) developed from multicellular pollen grains were examined in rice anther culture (Oryza sativa L.). The ultrastructure of polle...
Morphological details of rice callus cultures were examined at the initial stage of plantlet development, together with the growth of callus established from mature seeds, with the aid of scanning elc...
The formation of pollen callus was induced when rice anthers, which contained microspores in the mid-uninucleate and late-uninucleate stage, were cultured. The ultrastructure of the microspores was al...
High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were release...
Callus was initiated from mature seeds without the rinds of barnyard grass, Echinochloa oryzicola Vasing, on a modified Murashige and Skoog (MS) medium containing 2.0∼20.0 mgl-1 2, 4-D. The rate...
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....
This study was carried out to verify the production of haploid plantlets through somatic embryogenesis of Bupleurum falcatum in anther culture (2n=16). Flowers with anthers at the uninucleate stage, l...
We improved the aluminum borate whisker-mediated method of DNA delivery into rice callus (Oryza sativa L., cv. Notohikari). The following factors were examined: amount of whiskers, the kind of apparat...
Semithin and ultrathin sections of callus tissues of African rice (Oryza glaberrima Steud) after 7 and 14 days of initiation were compared with similar cultures after 3, 5, 7 and 10 days of incubation...
Some physiological changes induced by abscisic acid (ABA) were investigated in a long-term cultured callus of rice, Oryza sativa, L. cultivar YUHKARA. The results were as follows : (1) The retardation...
Histological observation was conducted to evaluate the origin, initiation site and development of callus in mungbean cotyledon culture. Results showed that callus formation in mungbean occurred in thr...
The callus induction potential of 15 indica rice genotypes from Indonesia was examined in comparison with that of the japonica rice Nipponbare. Callus was induced from embryos of mature seeds and root...
Callus is an excellent source for in vitro plant regeneration, but plants regenerated from callus sometimes show phenotypic and genotypic variation from the initial plants. In this study, the variatio...

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