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用农杆菌介导法将AtNHX1基因导入菊苣中,共获得42株卡那霉素(Kan)抗性再生植株。经过PCR 检测、Southern杂交和RT-PCR 检测表明,AtNHX1基因已成功整合到菊苣基因组中,并且能够正常转录。野生型和转基因植株诱发的愈伤组织进行耐盐生长试验,结果显示,相同盐胁迫条件下,转基因愈伤组织的相对生长率显著高于野生型愈伤组织。施加梯度NaCl胁迫后,植株叶片K+ 和Na+ 含量测定结果...
Ectopic Expression of AtNHX1 in Cotton (Gossypium hirsutum L.) Increases Proline Content and Enhances Photosynthesis under Salt Stress Conditions
Ectopic Expression AtNHX1 Cotton Proline Content Photosynthesis Salt Stress Conditions
2008/12/10
Overexpression of the Arabidopsis gene AtNHX1 that codes for a vacuolar Na+/H+ antiporter in transgenic cotton significantly improved tolerance to 200 mM NaCl as measured by increases in plant height,...