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河南省烟草疫霉遗传多样性与遗传结构的RAPD分析
烟草疫霉 随机扩增多态性DNA 遗传多样性 遗传结构
2021/4/7
为了明确河南省烟草疫霉群体的遗传多样性与遗传结构，利用RAPD分子标记对河南省的34个烟草疫霉菌株进行分析。结果表明，6条RAPD引物共扩增出30个条带，多态性条带比率为90%；Nei’s基因多样性指数为0.271 0，Shannon信息指数为0.402 8；菌株间的遗传相似系数在0.59~1.00，相似系数0.80水平下UPGMA聚类可将34个菌株划分为6个类群，类群Ⅰ包括26个菌株，为优势类群...
Mycoplasma gallisepticum strains with identical random amplified polymorphic DNA (RAPD) patterns in chukar partridges (Alectoris chukar) and broilers: a case report
RAPD Mycoplasma gallisepticum chukar partridge broiler
2015/4/17
We used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique to discriminate the major emerging poultry pathogen, Mycoplasma gallisepticum (MG), in broiler and chukar pa...
RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis
all-male bulked segregant analysis RAPD sex marker supermale
2015/3/17
Asparagus (Asparagus officinalis L.) plants are dioecious. All-male cultivars are desired because of their higher yields. To increase the proportion of male individuals planted in the field and expedi...
Genetic Variation of Chilo suppressalis Walker (Lepidoptera: Pyralidae) Populations in Guilan and West of Mazandaran Provinces analysed with RAPD Markers
RAPD PCR rice cultivate Rice striped stem borer
2015/3/11
Genetic variation of striped stem borer Chilo suppressalis populations in Guilan and Mazandaran provinces in Iran was
studied in 2010 by means of RAPD markers. Collected 45 samples from 19 locations...
RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey
quince genetic resource molecular markers
2015/3/4
Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost...
Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats
random amplified polymorphic DNA cattle sheep and goats typing
2015/2/5
n this study, 30, 15 and 1 strains of Pasteurella multocida and 9, 8 and 6 strains of Mannheimia haemolytica from cattle, sheep and goats isolated in Elazig province located in the East ...
Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique
Gliocladium spp. Gliocladium virens Paecilomyces fumosoroseus Verticillium lecanii entomopathogenic fungi mycoparasitic fungi identification using RAPD-PCR
2015/1/28
Entomopathogenic and mycoparasitic fungi were characterised by RAPD technique, with special attention to evaluate the genetic stability of strains that are used as active ingredients in commercial bio...
The use of RAPD markers for differentiation of grapevine varieties registered in the Czech Republic
grapevine registered cultivars
2015/1/21
Fifty-one grapevine cultivars registered in the Czech Republic were a subject of RAPD analysis. Nine grapevine varieties which were supposed to be genetically distant, were analysed by means of 120 Op...
The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones
clones fingerprinting RAPD
2015/1/21
Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris ...
RAPD analysis for genetic diversity of germplasm resources of Strobilanthes
Strobilanthes cusia (Nees) O. Ktze RAPD Germplasm Resources Genetic Diversity
2013/2/28
The 18 samples representing 18 populations of Strobilanthes cusia (Nees) O. Ktze in Fujian Province of China were analyzed with RAPD markers. Eleven primers were used, a total of 106 bands were scored...
广西沿海及其邻近海区拟穴青蟹群体遗传多样性的RAPD分析
拟穴青蟹 RAPD 遗传多样性 遗传分化
2012/8/13
采用随机扩增多态DNA（random amplified polymorphic DNA，RAPD）技术检测广西沿海及其邻近海区拟穴青蟹（Scylla paramamosain）6个地理群体的遗传变异和遗传结构，8条10 bp寡核苷酸随机引物扩增99个个体，分析其中的44个位点，31个位点表现出多态性，在种水平的多态位点百分率为70.45%。POPGENE分析结果显示，6个群体的多态位点百分率为2...
为深入研究并充分利用野生苦豆子（Sophora alopecuroides）资源，本研究利用RAPD技术对22个苦豆子居群进行研究，从DNA水平探讨苦豆子种质资源的遗传多样性。结果表明，筛选得到的31条RAPD随机引物共检测出314条扩增DNA片段，其中多态性条带285条，多态性比率为90.76%。Nei’s遗传多样性指数（H）为0.301 5，Shannon信息指数（I）为0.453 1，遗传距...
甘肃新选育党参品系与主栽品种的RAPD分析
党参 RAPD 遗传多样性 多态位点
2013/8/7
采用RAPD分子标记技术，分析了新选育党参（Codonopsis pilosula）8个品系和2个主栽品种的遗传多样性和亲缘关系。结果显示，14条引物共扩增出159个位点，其中多态位点26个，多态性位点比率为46.43%，材料间遗传相似系数范围在0.771 9～0.964 9，说明甘肃省主栽党参品种资源具有较丰富的遗传多样性。根据聚类分析结果，党参品种DS6与所有其他供试材料的亲缘关系最远，可单独...
从50条Random Amplified Polymorphic DNAs(RAPD)随机引物中选取多态扩增性强的15条引物,对蓟柄锈菌(Puccinia obtegens)的22个样品和3个大蓟上采集的锈病菌样品基因组DNA进行扩增,并构建指纹图谱。25个样品包括来自不同地区的9个夏孢子样品,不同孢子类型的6个样品,同一地点不同采集时间的7个夏孢子样品和3个大蓟上采集的样品。结果显示,扩增共产生...