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Correlation between the Degree of Hydrolysis and the Peptide Profile of Whey Protein Concentrate Hydrolysates: Effect of the Enzyme Type and Reaction Time
Hydrolysis Peptide Profile
2016/6/2
The use of protein hydrolysates for the development of new food products requires their prior characterization including the determination of the degree of hydrolysis (DH) and the distribution of pept...
通过酶法制备进行大米抗氧化肽蛋白酶的筛选。以大米蛋白为原料、酶解液对DPPH自由基清除率为指标，从风味蛋白酶、中性蛋白酶、胰蛋白酶、复合蛋白酶、碱性蛋白酶、木瓜蛋白酶和胃蛋白酶等7种蛋白酶中，筛选出中性蛋白酶为最佳用酶。通过试验得出最佳的酶解反应条件为：底物质量浓度1.0 g/(100mL)、加酶量8%、pH值6.0、温度60℃、酶解时间30min。酶解液稀释15倍对DPPH自由基的清除率为89...
马铃薯渣蛋白抗氧化肽的酶法制备（Enzymatic Preparation of Anti-oxidation Peptide from Potato Pulp）
马铃薯渣 抗氧化肽 酶解 DPPH自由基清除率
2010/12/28
以马铃薯渣为原料，采用酶法将薯渣中的蛋白转化为具有抗氧化活性的多肽。以酶解液对DPPH自由基清除率为酶解效果评价指标，从木瓜蛋白酶、风味蛋白酶、中性蛋白酶、碱性蛋白酶、胰蛋白酶、胃蛋白酶等6种商业蛋白酶中筛选出胰蛋白酶为最佳水解用酶。通过优化试验，得出薯渣蛋白最佳酶解条件为：底物质量浓度4g/（100mL）、加酶量7%、pH值8.0、料液温度50℃、酶解时间90min。酶解液稀释20倍后对DP...
Purification and Characterization of Antioxidant Peptide from Sunflower Protein Hydrolysate
antioxidant peptide sunflower protein hydrolysate purification and characterization
2011/2/24
Sunflower proteins were hydrolyzed with Flavourzyme for the production of antioxidant
peptide. DEAE-Sepharose Fast Flow, Sephadex G-25 gel filtration chromatography
and reversed-phase HPLC were cons...
An efficient parallelization of phosphorylated peptide and protein identification
peptide protein identification
2010/12/20
Protein sequence database search based on tandem mass spectrometry is an essential method for
protein identification. As the computational demand increases, parallel computing has become an important...
Speeding up Tandem Mass Spectrometry Based Database Searching by Peptide and Spectrum Indexing
Mass Spectrometry Database Searching Peptide Spectrum Indexing
2010/12/21
Database searching is the technique of choice for shotgun proteomics, and to date much research effort has been spent on improving its effectiveness. However, database searching faces a serious challe...
pNovo: De novo Peptide Sequencing and Identification Using HCD Spectra
Peptide Sequencing dentification HCD Spectra
2010/12/21
De novo peptide sequencing has improved remarkably in the past decade as a result of better
instruments and computational algorithms. However, de novo sequencing can correctly interpret only ∼3...
Emulsifying Properties of a Peptide from Peptic Hydrolysates of Soy Glycinin
glycinin pepsin peptide emulsifying activity
2009/6/10
Soy glycinin was hydrolyzed by pepsin under controlled conditions. The emulsifying activity of the hydrolysates was found to be higher than that of glycinin. Fractionation of the peptides was performe...
The FEL Irradiation Effects against a Phosphorylated Peptide and the Infrared Spectrographic Distinguishing Method of a Phosphate Group
Free Electron Laser (FEL) Phosphorylation and dephoosphorylation Fourier transform infrared Spectrometer (FT-IR)
2009/6/8
Phosphorylation and dephosphorylation that are most remarkable posttranslational modifications are important to the activation and deactivation of protein in living system. In the first approach, we s...
Seasonal Evolution of Hydrophilic and Hydrophobic Peptide Contents in Cheeses Made from Ewe’s Goat’s or Cow’s Milk
proteolysis goats ewes cows cheese
2014/3/10
Proteolysis is the principal and most complex biochemical event occurring during the maturation of the majority of ripened cheese varieties. In addition to softening the cheese body, proteolysis influ...
Efficient discovery of abundant post-translational modifications and spectral pairs using peptide mass and retention time differences
Efficient discovery abundant post-translational modifications spectral pairs using peptide mass retention time differences
2013/7/16
Background: Peptide identification via tandem mass spectrometry is the basic task of current proteomics research. Due to the complexity of mass spectra, the majority of mass spectra cannot be interpre...
PeakSelect: preprocessing tandem mass spectra for better peptide identification
preprocessing tandem mass spectra peptide identification
2010/12/17
We present a new preprocessing method, PeakSelect, to improve the accuracy and efficiency of
Tandem Mass-Spec peptide (protein) identification. The fundamental difference between noise and fragment i...
Efficient discovery of abundant post-translational modifications and spectral pairs using peptide mass and retention time differences
post-translational modifications spectral pairs peptide mass
2010/12/20
Peptide identification via tandem mass spectrometry is the basic task of current
proteomics research. Due to the complexity of mass spectra, the majority of mass spectra cannot be interpreted at pres...
Purification and Characterization of an Antihypertensive Peptide from a Yogurt-Like Product Fermented by Lactobacillus helveticus CPN4
yogurt-like product Lactobacillus helveticus antihypertensive peptide angiotensin I-converting enzyme
2008/3/28
Whey peptides in a yogurt-like product fermented by Lactobacillus helveticus CPN4 were fractionated by a Sep-pak C-18 cartridge followed by two-step reverse-phase HPLC. The antihypertensive activity w...
Properties of a Heparin-binding Peptide Derived from Bovine Lactoferrin
lactoferrin heparin binding conformation
2008/3/28
A heparin-binding peptide was isolated from a proteolytic hydrolysate of bovine lactoferrin by affinity chromatography using an immobilized heparin column. Analysis of amino acid sequences at the N-te...