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本研究基于全基因组分析,在雷蒙德氏棉、亚洲棉、海岛棉和陆地棉基因组中分别鉴定得到16、16、31和29个NF-YA基因。通过进化树分析将NF-YA基因分为4个亚组。结构分析表明NF-YA基因在棉属进化过程中相对保守。荧光定量显示GhNF-YAs可能通过ABA和 MeJA的调控通路响应盐和干旱。VIGS沉默GhNF-YA10和GhNF-YA23的表达,能显著降低棉花对盐的抗性,表明这两个基...
本研究利用收集的436份国内外变异广泛的陆地棉材料,采用全基因组重测序方法,构建了群体的SNP变异图谱,通过对包含136份早熟棉资源在内的356份陆地棉材料进行深度重测序,并结合公共数据库中的80份陆地棉材料共获得了10,118,884个高质量的SNPs。利用全基因组关联分析(GWAS)鉴定到控制陆地棉早熟性的307个显著遗传位点和重要调控基因。两个早熟遗传热点区域位于 D03和A05&...
Reverse transcription quantitative real-time PCR (RT-qPCR) is widely used to study the relative abundance of mRNA transcripts because of its sensitivity and reliable quantification. However, the reli...
Gene regulatory networks capture interactions between genes and other cell substances, resulting in various models for the fundamental biological process of transcription and translation. The expressi...
Gene regulatory networks capture interactions between genes and other cell substances, resulting in various models for the fundamental biological process of transcription and translation. The expressi...
Leaf rust (LR), caused by Puccinia triticina (Pt), is worldwide one of the most spread diseases in common wheat (Triticum aestivum L.). With the objective of identifying and mapping new genes for re...
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
As biomedical research and advances in biotechnology generate expansive datasets, the need to process this data into information has grown simultaneously. Specifically, recognizing and extracting thes...
This dissertation examines the molecular mechanisms regulating Rac2 gene expression during cell differentiation and identification of a minimal cis-element required for the induction of Rac2 gene expr...
The heart is the first organ to form during development in vertebrates and Nkx2-5 is the first marker of cardiac specification. In Xenopus laevis, Nkx2-5 is essential for heart formation, but early ta...
Friedreich’s Ataxia (FRDA) is a rare and poorly understood autosomal recessive disease caused by a pathological deficiency of the mitochondrial protein frataxin. Patients suffer neurodegeneration, ata...
Fetal alcohol exposure is the leading known cause of mental retardation in the western world. However, the mechanisms underlying alcohol-induced damage in fetal brain are largely unknown. The goal of ...
The proteasome is responsible for breaking down the majority of the proteins in the cell. However, a complete understanding of how this large multi-subunit protease is assembled is currently lacking. ...
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...

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