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Use of murine models to test novel gene transfer strategies for the treatment of Fanconi anemia
Gene therapy mobilization hematopoiesis
2015/5/21
he dawn of the genetic era has allowed for investigation of gene transfer therapy as a treatment for certain diseases. Fanconi anemia (FA) is a rare genetic disorder in which the majority of patients ...
Statistical Mechanics of Horizontal Gene Transfer in Evolutionary Ecology
Statistical Mechanics Horizontal Gene Transfer Evolutionary Ecology
2011/1/5
The biological world, especially its majority microbial component, is strongly interacting and may be dominated by collective effects. In this review, we provide a brief introduction for statistical p...
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...
水平基因转移Horizontal Gene Transfer
水平基因转移 进化与系统发育 基因工程生物安全性
2007/12/7
摘要简要介绍了水平基因转移（horizontal gene transfer,HGT）的基本概念以及进行转移的主要方式：由质粒或病毒等介导的水平基因转移和基因的“直接”水平转移。并结合基因组序列分析，重点阐述远缘生物之间发生的广泛的基因交流，以及其与进化、系统发育和基因工程生物安全性问题的探讨。
Abstract:In this paper the conception of horizonta...
精子为载体的基因转移研究--Research on Gene Transfer by Spermatozoa
精子 转基因载体 脂质体 spermatozoa transgenic vector lipsome
2007/12/5
采用共培养和脂质体介导两种方法将金黄地鼠附睾内的精子与NFκB-luc+质粒共孵育后，再与成熟的卵母细胞进行体外受精，用PCR、Southern blot及荧光原位杂交(FISH)等技术检测外源基因在精子细胞和在受精卵中的存在及存在部位，并对相关数据进行统计学比较分析。结果表明，金黄地鼠附睾内的活精子具有主动捕获外源基因的能力，而且外源基因只存在于精子细胞的头部，而且通过受精将外源基因带入了卵细胞...
期刊信息
篇名
Adenovirus-Mediated Ink4a/ARF Gene Transfer Significantly suppressed the growth of pancreatic carcinoma cells
语种
英文
撰写或编译
撰写
作者
Fang Chen,Yiting Li,Zhaohui Lu,Jie Gao,Jie Chen
第一作者单位
中国医学科学院中国...
Application of Gene Transfer Technology for Genetic Improvement of Fish
Transgenic fish genetic improvement of fish gene transfer aquaculture
2010/3/29
The worldwide decline of natural fish stocks, the accumulation of chemical pollutants in aquatic environments and increasing world consumption of fish products has provided an impetus in many countrie...
Follow-up of Exogenous DNA by Sperm-mediated Gene Transfer via Liposome
Gene Transfer Sperm Vector Intracytoplasmic Sperm Injection Mouse Bacterial Lacz
2016/4/12
To examine the feasibility of using a sperm vector system for gene transfer, we have investigated the binding and the uptaking of foreign DNA into the sperm nucleus by PCR, in situ hybridization and L...
Evaluation of Various Sunflower ( Helianthus annuus L.) Genotypes for Agrobacterium tumefaciens-mediated Gene Transfer
Sunflower gene transfer Agrobacterium tumefaciens genotypes
2010/3/23
In this work, 10 sunflower ( Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium tumefaciens-mediated gene transfer based on detection of a reported gene expression ...