搜索结果: 1-15 共查到“生物学 Spermatozoa”相关记录15条 . 查询时间(0.072 秒)
Species Difference in the Expression of Fas and Fas Ligand in Mature Mammalian Spermatozoa
sperm Fas antigen FasL apoptosis mammals
2018/3/5
Li Y., Sun M., Zhu J., Jiao G., Lin J., Dong H., Tan J., Zhou J. (2017): Species difference in the expression of
Fas and Fas ligand in mature mammalian spermatozoa. Czech J. Anim. Sci., 62, 519–526.
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Development and application of LC/HRPS for quantification of adenine nucleotides,creatine phosphate,and creatine in sturgeon spermatozoa
macroergic phosphates high resolution mass spectrometry extraction method validation sperm chondrostean fishes
2017/2/21
The objective of this study was to investigate the applicability of liquid chromatography coupled with high resolution product scan (LC/HRPS) mass spectrometry for simultaneous quantification of adeni...
Low-density Lipoprotein Improves Motility and Plasma Membrane Integrity of Cryopreserved Canine Epididymal Spermatozoa
Low-density Lipoprotein Epididymal Spermatozoa Cryopreservation Dog
2016/5/20
Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg ...
Effects of Hormones on the Expression of Matrix Metalloproteinases and Their Inhibitors in Bovine Spermatozoa
Bovine Spermatozoa MMPs TIMPs Capacitation
2016/5/11
Proteases and protease inhibitors play key roles in most physiological processes, including cell migration, cell signaling, and cell surface and tissue remodeling. Among these, the matrix metalloprote...
Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
SRY Gene Bovine Spermatozoa In situ Hybridization Bovine
2016/5/9
The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR expe...
Effects of Butylated Hydroxytoluene on Freezability of Ram Spermatozoa
Butylated Hydroxytoluene Ram Spermatozoa Freezability
2016/5/5
The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected...
Effects of Sucrose and Trehalose on the Freezability of Markhoz Goat Spermatozoa
Markhoz Goat Cryopreservation Semen Trehalose Sucrose
2016/5/3
The present study was conducted to observe the effect of increased osmolality of basic tris extender supplemented with trehalose and sucrose on post-thawing quality (motility, progressive motility, vi...
Effect of Antioxidant Preservative on Cold Protection Ability of Low Grade Riverine Buffalo (Bubalus bubalis) Bull Spermatozoa
Buffalo Semen Sperm Motility Non-Eosinohilic Butylated Hydroxy Anisole Butylated Hydroxy Toluene Pentoxifylline Theophylline Theobromine
2016/4/29
An experiment was conducted to investigate the effect of Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX), Theophylline (TPY) and Theobromine (TBR) on cold protec...
Effects of Ca2+ and HCO3- on Capacitation, Hyperactivation and Protein Tyrosine Phosphorylation in Guinea Pig Spermatozoa
Capacitation Hyperactivation Tyrosine Phosphorylation Sperm
2016/4/29
In our previous report, we demonstrated that the tyrosine phosphorylation of sperm proteins (TPSP) of guinea pig was associated with capacitation and hyperactivation (CAHA), and Ca2+ and HCO3- were re...
Effects of Sucrose and Glycerol during the Freezing Step of Cryopreservation on the Viability of Goat Spermatozoa
Goat Spermatozoa Cryopreservation Osmolality Sucrose Glycerol
2016/4/28
Four experiments were conducted to study the following: i) the influence of different concentrations of sucrose (0.15, 0.3 and 0.5 M with osmolality of 308, 500 and 760 mOsm/kg, respectively) in dilue...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...
精子为载体的基因转移研究--Research on Gene Transfer by Spermatozoa
精子 转基因载体 脂质体 spermatozoa transgenic vector lipsome
2007/12/5
采用共培养和脂质体介导两种方法将金黄地鼠附睾内的精子与NFκB-luc+质粒共孵育后，再与成熟的卵母细胞进行体外受精，用PCR、Southern blot及荧光原位杂交(FISH)等技术检测外源基因在精子细胞和在受精卵中的存在及存在部位，并对相关数据进行统计学比较分析。结果表明，金黄地鼠附睾内的活精子具有主动捕获外源基因的能力，而且外源基因只存在于精子细胞的头部，而且通过受精将外源基因带入了卵细胞...
Influence of Osmolality of Complete Semen Extender on Motion Characteristics of Frozen-thawed Ram Spermatozoa
Sheep Extender Osmolality Spermatozoa Cryopreservation Computer-aided Semen Analysis
2016/4/22
The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintai...
Cock Spermatozoa Serve as the Gene Vector for Generation of Transgenic Chicken (Gallus gallus)
Transgenic Chicken Sperm-mediated Gene Transfer
2016/4/15
To evaluate the feasibility of using sperm-mediated gene transfer (SMGT) for carrying foreign gene into chicken oocyte, a reporter gene, CX-EGFP, was used in this study. The reporter gene was first mi...
Arsenic Toxicity on Duck Spermatozoa and the Ameliorating Effect of L-Ascorbic Acid
Drake Sperm Quality Antioxidant Ascorbic Acid Fertilization Rate LD50
2016/4/8
The objectives of this study were to understand the possible mechanism of duck sperm toxicity induced by arsenic exposure in vivo, and to investigate the roles of the antioxidant L-ascorbic acid in am...