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Immunohistochemical localization of adhesion molecules (CD62 and CD18) in the mammary gland of dairy cows
cattle L-selectin β2-integrin mastitis
2015/6/4
Localization of the L-selectin (CD62L) and β2-integrin (CD18) bearing cells in different tissues of the bovine mammary gland was examined. Five dairy cows of Holstein-Friesian breed in the middle of t...
Regulation of the p53 tumor suppressor gene in the mammary gland and its role in tumorigenesis
Biological sciences p53 Tumor suppressor Mammary gland Tumorigenesis
2015/1/28
Breast cancer is the most frequent tumor type among women. Heightened susceptibility of the breast to tumor development has been associated with early menarche, nulliparity, exposures to ionizing radi...
Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx
bovine virgin mammary gland induced influx
2015/1/20
Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of...
Alternative splicing of MDM2 during breast tumorigenesis and mammary gland *development
Genes proliferation cell cycle growth abnormal genes oncogenes and tumor suppressor protein and tumor
2014/12/11
The regulation of genes involved in proliferation and cell cycle control plays a critical role in normal development and differentiation. Aberrant expression of genes promoting proliferation (oncogene...
Effects of Long Term Exogenous Bovine Somatotropin on Nutrients Uptake by the Mammary Gland of Crossbred Holstein Cattle in the Tropics
rbST Nutrients Mammary Gland Uptake Crossbred Holstein Cattle
2016/5/3
Ten, first lactation, 87.5%HF dairy cattle were used to investigate effects of long-term administration of recombinant bovine somatotropin (rbST) on nutrient uptake by the mammary gland at different s...
催乳素和外源基因乳腺共表达载体可提高外源基因在哺乳细胞的表达Mammary Gland Dual－Expression Construct Containing Prolactin and Foreign DNAs Can Promote the Expression of Foreign Proteins in Mammary Cells
催乳素 乳铁蛋白 血小板生成素 表达
2008/1/8
摘要
为探讨应用牛催乳素促进乳腺生物反应器高效表达外源蛋白（如人乳铁蛋白、人血小板生成素）的可行性，构建两种牛催乳素和外源基因乳腺共表达载体。一种为牛催乳素基因和外源基因共用一个6.7 kb山羊β-酪蛋白启动子启动转录，两个基因以内部核糖体进入位点(IRES)连接；另一种为外源基因利用6.7 kb山羊β－酪蛋白启动子，催乳素基因利用2.0 kb山羊β-酪蛋白启动子，分别转录。脂质体转染后，RT...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白（β-lactoglobulin,BLG）基因，扩增出的两个片段分别克隆到T载体上，利用BLG基因序列自身存在的NarI单酶切位点进行拼接，获得了全长为7.2kb的山羊BLG基因克隆，并构建了它的真核表达载体，经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠，经PCR和Southern印迹分析...
Effects of Long Term Exogenous Bovine Somatotropin on Nutrients Uptake by the Mammary Gland of Crossbred Holstein Cattle in the Tropics
rbST Nutrients Mammary Gland Uptake Crossbred Holstein Cattle
2016/4/26
Ten, first lactation, 87.5%HF dairy cattle were used to investigate effects of long-term administration of recombinant bovine somatotropin (rbST) on nutrient uptake by the mammary gland at different s...
Expression of B Cell Activating Factor Pathway Genes in Mouse Mammary Gland
B Cell Activating Factor APRIL Apoptosis Mammary Gland
2016/4/25
In our previous study, overexpression of extracellular proteinase inhibitor (Expi) gene accelerated apoptosis of mammary epithelial cells, and induced expression of B cell activating factor (BAFF) gen...
Functional Characterization of Mammary Gland of Holstein Cows under Humid Tropical Summer Climates
Tropics Holstein Cow Lactation N-Acetylglucosaminidase Urea
2016/4/13
Physiological parameters were measured on six primiparous, non-pregnant Holstein cows prior to peak lactation over a 3-month summer season in southwestern Taiwan. The objectives were to characterize h...
Effects of Controlled Compensatory Growth on Mammary Gland Development and Lactation in Rats
Compensatory Growth Mammary Gland Lactation Cell Differentiation Rats
2016/4/11
The objective of this study was to examine the effect of compensatory growth nutritional regimen on mammary gland growth and lactation. One hundred twenty-two Sprague Dawley female rats (35 days of ag...
Expression of Stat5a Gene in Bovine Mammary Gland and its Effect on Proliferation of Mammary Epithelial Cells
Bovine Mammary Tissues Stat5a Pregnant Cell Proliferation
2016/4/11
To understand molecular mechanisms involved in bovine mammary gland growth, expression of stat5a gene was examined in bovine mammary tissues. We found that stat5a gene was highly induced at pregnant 7...
Effects of Active Immunization against Somatostatin or its Analogues on Milk Protein Synthesis of Rat Mammary Gland Cells
Somatostatin Active Immunization GH Concentration Milk Protein Synthesis
2016/4/8
Effects of active immunization against native 14-mer somatostatin (SRIF, somatotropin releasing inhibiting factor) and its two 14-mer-somatostatin analogues on the milk production in rat mammary cells...