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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Liquid chromatography/mass spectrometry analysis of perfluoroalkyl carboxylic acids and perfluorooctanesulfonate in bivalve shells: Extraction method optimization
Perfluorinated carboxylic acids Perfluorooctanesulfonate Shell Soft tissue Acid digestion Solid phase extraction
2013/12/12
Different extraction methods, including extraction by organic solvents with and without acetic acid digestion,and mixed inorganic acid digestion coupled with solid phase extraction (SPE), were develop...
Retention Behavior on Aminoethyl-modified Poly(p-phenylene terephthalamide) Fiber Stationary Phases in Gas Chromatography
Retention Behavior Aminoethyl-modified Poly(p-phenylene terephthalamide)
2010/9/17
Surface derivatization of Kevlar, poly(p-phenylene terephthalamide), fiber has been studied along with the evaluation of the surface characteristics of the chemically-modified fiber as the stationary ...
Trace Analysis of Polycyclic Aromatic Hydrocarbons Using Gas Chromatography–Mass Spectrometry Based on Nanosecond Multiphoton Ionization
Trace Analysis Polycyclic Aromatic Hydrocarbons Gas Chromatography–Mass Spectrometry Based Nanosecond Multiphoton Ionization
2010/9/15
Gas chromatography/resonance-enhanced multiphoton ionization/time-of-flight mass spectrometry (GC/REMPI-TOFMS) using an ultraviolet nanosecond laser was employed in the trace analysis of polycyclic ar...
Multifunctional Separation Mechanism on Poly(oxyethylene) Stationary Phases in Capillary Liquid Chromatography
Multifunctional Separation Mechanism Capillary Liquid Chromatography
2010/9/17
The retention behavior on poly(oxyethylene) (POE) chemically-bonded stationary phases was investigated in ion-exchange, reversed-phase (RP) and hydrophilic interaction (HILIC) modes in capillary liqui...
Mesoporous Platinum Electrodes for Amperometric Determination of Sugars with Anion Exchange Chromatography
Mesoporous Platinum Electrodes Amperometric Determination Sugars
2010/9/17
A simple amperometric detection (AD) method based on mesoporous platinum (Pt) electrodes has been developed for the determination of sugars (glucose, fructose, and sucrose) with high-performance anion...
Separation of Optical Isomers in Capillary Chromatography Using a Poly(tetrafluoroethylene) Capillary Tube and an Aqueous–Organic Mixture Carrier Solution
Separation Optical Isomers in Capillary Chromatography tetrafluoroethylene
2010/9/13
Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water–hydrophilic/hydrophobic organic solvent mixture as ...
Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography
Temperature-Programmed Separation Retention Prediction Novel Packed-Capillary Column
2010/9/13
Novel packed-capillary columns for gas chromatography were developed with a thin-wall stainless-steel capillary of 1.0 mm i.d.; and rapid temperature-programmed separations have been carried out after...
Rapid and Simultaneous Determination of Tetrafluoroborate, Thiocyanate and Hexafluorophosphate by High-Performance Liquid Chromatography Using a Monolithic Column and Direct Conductivity Detection
Rapid Simultaneous Determination Tetrafluoroborate
2010/9/15
A method was developed for fast and simultaneous determination of tetrafluoroborate (BF4−), thiocyanate (SCN−) and hexafluorophosphate (PF6−) by high-performance liquid chromatograph...
Analytical Conditions and Separation Performance of Capillary Chromatography Based on the Tube Radial Distribution of Aqueous–Organic Mixture Carrier Solvents under Laminar-Flow Conditions
Analytical Conditions Separation Performance Capillary Chromatography Based
2010/9/14
We have developed a capillary chromatography system using an open capillary tube made of fused-silica, polyethylene, or poly(tetrafluoroethylene), and a water–hydrophilic–hydrophobic organic mixture c...
Analyses of Non-steroidal Anti-inflammatory Drugs in Environmental Water Samples with Microemulsion Electrokinetic Chromatography
Non-steroidal Anti-inflammatory Drugs Environmental Water Samples Microemulsion Electrokinetic Chromatography
2010/9/13
In this study, a microemulsion electrokinetic chromatography (MEEKC) was used to analyze ten non-steroidal anti-inflammatory drugs (NSAIDs) in water samples. The type and ratio of organic modifiers we...
Analysis of Risedronate and Related Substances by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection
Analysis Ion-Pair Reversed-Phase High-Performance Liquid Chromatography Light-Scattering Detection
2010/9/17
A simple method has been developed for the analysis of risedronate and related substances by ion-pair reversed-phase high-performance liquid chromatography (RPLC) with evaporative light-scattering det...
Measurement of Transport Activities of Bile Acids in Human Multidrug Resistance-Associated Protein 3 Using Liquid Chromatography–Tandem Mass Spectrometry
Transport Activities Bile Acids Human Multidrug Resistance-Associated Protein 3 Liquid Chromatography–Tandem Mass Spectrometry
2010/9/17
A method has been developed for the measurement of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 3-expressing Sf9 cells for 1β-hydroxy-, 6α-hydr...
Simultaneous Determination of Estradiol Valerate and Medroxyprogesterone Acetate in a Tablet Formulation by Gas Chromatography–Mass Spectrometry
Estradiol Valerate Medroxyprogesterone Acetate Gas Chromatography–Mass Spectrometry
2010/9/19
A new and simple gas chromatography–mass spectrometry (GC-MS) method has been developed for simultaneous determination of estradiol valerate (EV) and medroxyprogesterone acetate (MPA) in a tablet form...
Simultaneous Determinations of Cr(VI) and Cr(III) by Ion-Exclusion/Cation-Exchange Chromatography with an Unmodified Silica-Gel Column
Cr(VI) Cr(III) Ion-Exclusion/Cation-Exchange Chromatography an Unmodified Silica-Gel Column
2010/9/19
In order to characterize the ion-exclusion and cation-exchange properties of an unmodified silica-gel column, the retention behaviors of Cr(VI) and Cr(III) ions were investigated using a Develosil 30-...