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Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
Genotyping Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes PCR-RFLP Dried Blood DNA Extraction
2010/9/20
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
DNA提取的应用与相关技术分析Application of DNA Extraction and Analysis of Its Influence Factors
DNA提取 血液 新鲜组织 石蜡标本 冰冻组织
2007/12/30
摘要为了分析影响提取DNA的有关因素,采用标准酚—氯仿抽提法和蛋白酶消化法提取DNA。结果表明,平均每mL全血可提取200 ~ 300μgDNA;新鲜组织及OCT包埋冰冻组织提取DNA,平均每0.2g组织可获得200~300μgDNA;直接将石蜡标本提取物制作模版,PCR扩增良好。从4种组织中均获得较为纯净的DNA;OCT对组织DNA无不良影响。
Abstract:To explore infl...
期刊信息
篇名
An efficient protocol for genomic DNA extraction from citrus species
语种
英文
撰写或编译
作者
Cheng YJ,Guo WW,Yi HL,Pang XM,Deng XX
第一作者单位
刊物名称
Plant Molecular Biology Reporter
页面
21(2): 177-178 (2003
出...